SECURITIES AND EXCHANGE COMMISSION

-----BEGIN PRIVACY-ENHANCED MESSAGE----- Proc-Type: 2001,MIC-CLEAR Originator-Name: webmaster@www.sec.gov Originator-Key-Asymmetric: MFgwCgYEVQgBAQICAf8DSgAwRwJAW2sNKK9AVtBzYZmr6aGjlWyK3XmZv3dTINen TWSM7vrzLADbmYQaionwg5sDW3P6oaM5D3tdezXMm7z1T+B+twIDAQAB MIC-Info: RSA-MD5,RSA, WvReXHJnIryLOotV6tZ/M+5PgDMzkTGTZh8G9OY2yrxbOXK55m8uUs743MF6grFn jkB9UL+Od+6/H5gzE+KCZQ== 0001354488-10-000348.txt : 20100216 0001354488-10-000348.hdr.sgml : 20100215 20100216062257 ACCESSION NUMBER: 0001354488-10-000348 CONFORMED SUBMISSION TYPE: SC 13G/A PUBLIC DOCUMENT COUNT: 2 FILED AS OF DATE: 20100216 DATE AS OF CHANGE: 20100216 SUBJECT COMPANY: COMPANY DATA: COMPANY CONFORMED NAME: APPLIED NEUROSOLUTIONS INC CENTRAL INDEX KEY: 0000872947 STANDARD INDUSTRIAL CLASSIFICATION: BIOLOGICAL PRODUCTS (NO DIAGNOSTIC SUBSTANCES) [2836] IRS NUMBER: 391661164 STATE OF INCORPORATION: DE FISCAL YEAR END: 1231 FILING VALUES: FORM TYPE: SC 13G/A SEC ACT: 1934 Act SEC FILE NUMBER: 005-55419 FILM NUMBER: 10602202 BUSINESS ADDRESS: STREET 1: 50 LAKEVIEW PARKWAY S STREET 2: STE 111 CITY: VERNON HILLS STATE: IL ZIP: 60061 BUSINESS PHONE: 847 573 8000 MAIL ADDRESS: STREET 1: 50 LAKEVIEW PARKWAY STREET 2: STE 111 CITY: VERNON HILLS STATE: IL ZIP: 60061 FORMER COMPANY: FORMER CONFORMED NAME: HEMOXYMED INC DATE OF NAME CHANGE: 20020820 FORMER COMPANY: FORMER CONFORMED NAME: OPHIDIAN PHARMACEUTICALS INC DATE OF NAME CHANGE: 19970714 FILED BY: COMPANY DATA: COMPANY CONFORMED NAME: WILMINGTON TRUST CORP CENTRAL INDEX KEY: 0000872821 STANDARD INDUSTRIAL CLASSIFICATION: STATE COMMERCIAL BANKS [6022] IRS NUMBER: 510328154 STATE OF INCORPORATION: DE FISCAL YEAR END: 1231 FILING VALUES: FORM TYPE: SC 13G/A BUSINESS ADDRESS: STREET 1: RODNEY SQUARE NORTH STREET 2: 1100 NORTH MARKET ST CITY: WILMINGTON STATE: DE ZIP: 19890-0001 BUSINESS PHONE: 3026518378 MAIL ADDRESS: STREET 1: 1100 NORTH MARKET STREET CITY: WILMINGTON STATE: DE ZIP: 19890-0001 SC 13G/A 1 wl_sc13ga.htm AMENDMENT 1 SECURITIES AND EXCHANGE COMMISSION

Washington, D.C. 20549

SCHEDULE 13G

INFORMATION TO BE INCLUDED IN STATEMENTS FILED PURSUANT

TO RULES 13d-1(b), (c), AND (d) AND AMENDMENTS THERETO FILED

PURSUANT TO RULE 13d-2(b)

Amendment No. 1

Applied Neurosolutions Inc.

--------------------------------------------------------------------------------

(Name of Issuer)

Common Stock

--------------------------------------------------------------------------------

(Title of Class of Securities)

03823Y-203

--------------------------------------------------------------------------------

(CUSIP Number)

December 31, 2009

--------------------------------------------------------------------------------

(Date of Event Which Requires Filing of this Statement)

Check the appropriate box to designate the rule pursuant to which this Schedule is filed:

þ Rule 13d-1(b)

¨ Rule 13d-1(c)

¨ Rule 13d-1(d)

----------

* The remainder of this cover page shall be filled out for a reporting person's initial filing on this form with respect to the subject class of securities, and for any subsequent amendment containing information which would alter the disclosures provided in a prior cover page.

The information required in the remainder of this cover page shall not be deemed to be "filed" for the purpose of Section 18 of the Securities Exchange Act of 1934 ("Act") or otherwise subject to the liabilities of that section of the Act but shall be subject to all other provisions of the Act (however, see the Notes).

CUSIP No. 03823Y-203


1	NAME OF REPORTING PERSONS I.R.S. IDENTIFICATION NO. OF ABOVE PERSONS (ENTITIES ONLY) Wilmington Trust Corporation
2	CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP*
	(a)			ü
	(b)

3	SEC USE ONLY
4	CITIZENSHIP OR PLACE OF ORGANIZATION Delaware corporation
NUMBER OF		5	SOLE VOTING POWER 273,588
SHARES BENEFICIALLY OWNED BY		6	SHARED VOTING POWER 0
EACH REPORTING PERSON		7	SOLE DISPOSITIVE POWER 273,588
WITH		8	SHARED DISPOSITIVE POWER 0
9	AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH REPORTING PERSON 273,588
10	CHECK BOX IF THE AGGREGATE AMOUNT IN ROW (9) EXCLUDES CERTAIN SHARES*

	N/A
11	PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW (9) 6.2%
12	TYPE OF REPORTING PERSON* HC

CUSIP No. - 03823Y-203


1	NAME OF REPORTING PERSONS I.R.S. IDENTIFICATION NO. OF ABOVE PERSONS (ENTITIES ONLY) Wilmington Trust Company, in various fiduciary capacities
2	CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP*
	(a)			ü
	(b)

3	SEC USE ONLY
4	CITIZENSHIP OR PLACE OF ORGANIZATION Delaware banking corporation
NUMBER OF		5	SOLE VOTING POWER 273,588
SHARES BENEFICIALLY OWNED BY		6	SHARED VOTING POWER 0
EACH REPORTING PERSON		7	SOLE DISPOSITIVE POWER 273,588
WITH		8	SHARED DISPOSITIVE POWER 0
9	AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH REPORTING PERSON 273,588
10	CHECK BOX IF THE AGGREGATE AMOUNT IN ROW (9) EXCLUDES CERTAIN SHARES*


11	PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW (9) 6.2%
12	TYPE OF REPORTING PERSON* BK

CUSIP No. 03823Y-203

______________________________________________________________________________

Item 1(a). Name of Issuer:

Applied Neurosolutions, Inc.

______________________________________________________________________________

Item 1(b). Address of Issuer's Principal Executive Offices:

50 Lakeview Pkwy, Suite 111, Vernon Hills, IL 60061

_____________________________________________________________________________

Item 2(a). Name of Person Filing:

Wilmington Trust Corporation and Wilmington Trust Company

_____________________________________________________________________________

Item 2(b). Address of Principal Business Office, or if None, Residence:

1100 North Market Street

Wilmington, DE 19890

______________________________________________________________________________

Item 2(c). Citizenship:

Wilmington Trust Corporation is a Delaware corporation;

Wilmington Trust Company is a Delaware banking corporation.

______________________________________________________________________________

Item 2(d). Title of Class of Securities:

Common Stock

______________________________________________________________________________

Item 2(e). CUSIP Number:

03823Y-203

____________________________________________________________________

Item 3. If This Statement is Filed Pursuant to Rule 13d-1(b), or 13d-2(b) or (c), Check Whether the Person Filing is a:

(a) ¨ Broker or dealer registered under Section 15 of the Exchange Act.

(b) þ Bank as defined in Section 3(a)(6) of the Exchange Act.

Wilmington Trust Company is a Bank and is a direct, wholly- owned subsidiaries of Wilmington Trust Corporation.

CUSIP No. 03823Y-203

(c)

Insurance company as defined in Section 3(a)(19) of the Exchange Act.

(d)

Investment company registered under Section 8 of the Investment Company Act.

(e)

An investment adviser in accordance with Rule 13d-1(b)(1)(ii)(E);

(f)

An employee benefit plan or endowment fund in accordance with Rule 13d-1(b)(1)(ii)(F);

(g)

A parent holding company or control person in accordance with Rule 13d-1(b)(1)(ii)(G);

Wilmington Trust Corporation is a Parent Holding Company.

(h)

A savings association as defined in Section 3(b) of the Federal Deposit Insurance Act;

(i)

A church plan that is excluded from the definition of an investment company under Section 3(c)(14) of the Investment Company Act;

(j)

Group, in accordance with Rule 13d-1(b)(1)(ii)(J).

Wilmington Trust Corporation and Wilmington Trust Company.

Item 4. Ownership.

Provide the following information regarding the aggregate number and percentage of the class of securities of the issuer identified in Item 1.

(a) Amount beneficially owned: 273,588

(b) Percent of class:

6.2%


(i)	Sole power to vote or to direct the vote shares	273,588
(ii)	Shared power to vote or to direct the vote shares	0
(iii)	Sole power to dispose or to direct the disposition of shares	273,588
(iv)	Shared power to dispose or to direct the disposition of shares	0

Number of shares as to which Wilmington Trust Company has:


(i)	Sole power to vote or to direct the vote shares	273,588
(ii)	Shared power to vote or to direct the vote shares	0
(iii)	Sole power to dispose or to direct the disposition of shares	273,588
(iv)	Shared power to dispose or to direct the disposition of shares	0

______________________________________________________________________________

Item 5. Ownership of Five Percent or Less of a Class.

If this statement is being filed to report the fact that as of the date hereof the reporting person has ceased to be the beneficial owner of more than five percent of the class of securities, check the following ¨

______________________________________________________________________________

Item 6. Ownership of More Than Five Percent on Behalf of Another Person.

Not applicable.

______________________________________________________________________________

Item 7. Identification and Classification of the Subsidiary Which Acquired the Security Being Reported on by the Parent Holding Company.

Wilmington Trust Company:

______________________________________________________________________________

Item 8. Identification and Classification of Members of the Group.

Wilmington Trust Corporation:

Wilmington Trust Company:

______________________________________________________________________________

Item 9. Notice of Dissolution of Group.

Not applicable.

______________________________________________________________________________

Item 10. Certifications.

By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were acquired and are held in the ordinary course of business and were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect.

SIGNATURE

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.


February 12, 2010	WILMINGTON TRUST CORPORATION
	WILMINGTON TRUST COMPANY


	By:	/s/
		Gerard A. Chamberlain Assistant Secretary and Vice President

Attention. Intentional misstatements or omissions of fact constitute federal criminal violations (see 18 U.S.C. 1001).

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